Quantitative Detection of the Influenza a Virus by an EGOFET-Based Portable Device
Olena Yu. Poimanova, Elena Zavyalova, Elena A. Kretova, Антон А. Абрамов, Askold A. Trul, Oleg V. Borshchev, Anna Keshek, Sergey A. Ponomarenko, Elena V. Agina
Abstract
Elaboration of biosensors on the base of organic transistors with embedded biomolecules which can operate in an aqueous environment is of paramount importance. Electrolyte-gated organic field-effect transistors demonstrate high sensitivity in detection of various analytes. In this paper, we demonstrated the possibility of quantitative fast specific determination of virus particles by an aptasensor based on EGOFET. The sensitivity and selectivity of the devices were examined with the influenza A virus as well as with control bioliquids like influenza B, Newcastle disease viruses or allantoic fluid with different dilutions. The influence of the semiconducting layer thickness on EGOFETs sensory properties is discussed. The fabrication of a multi-flow cell that simultaneously registers the responses from several devices on the same substrate and the creation of a multi-sensor flow device are reported. The responses of the elaborated bioelectronic platform to the influenza A virus obtained with application of the portable multi-flow mode are well correlated with the responses obtained in the laboratory stationary mode.