Development of a horseradish peroxidase-nanobody fusion protein for visual detection of ochratoxin A by dot immunoassay
Qi Chen, Yuanyuan Wang, Fujing Mao, Benchao Su, Kunlu Bao, Zeling Zhang, Guifang Xie, Xing Liu
Abstract
in rice and oat samples by eye. The HN-DIA showed high selectivity for OTA and had good accuracy and reproducibility in the recovery experiments. Spiked sample analysis results of the HN-DIA and high performance liquid chromatography (HPLC) correlated well with each other. Therefore, the proposed HN-DIA has the potential for rapid screening of OTA and other small molecule pollutants in food and the environment by naked eye.
Topics & Concepts
Horseradish peroxidaseImmunoassayOchratoxin AChemistryPeroxidaseFusion proteinChromatographyBiochemistryAntibodyEnzymeFood scienceBiologyRecombinant DNAMycotoxinImmunologyGeneMycotoxins in Agriculture and FoodMicrobial infections and disease researchIdentification and Quantification in Food