Bacterial Growth Curve Measurements with a Multimode Microplate Reader
Ariel Rogers, Kaitlin R Bullard, Akash C Dod, Yong Wang
Abstract
Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution, higher efficiency, and are less laborious, while analysis and interpretation of the microplate-based measurements are less straightforward. Recently, we developed a new analysis method for evaluating bacterial growth with microplate readers based on time derivatives. Here, we describe a detailed protocol for this development and provide the homemade program for the new analysis method.
Topics & Concepts
CuvetteGrowth curve (statistics)PhotometerStandard curveBacterial growthChromatographyAnalytical Chemistry (journal)Biological systemChemistryOpticsBiologyStatisticsBacteriaMathematicsPhysicsGeneticsBiosensors and Analytical DetectionListeria monocytogenes in Food SafetyBacterial Identification and Susceptibility Testing