Survey of various carbapenem-resistant mechanisms of <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> isolated from clinical samples in Iran.
Leila Azimi, Fatemeh Fallah, Abdollah Karimi, Mehdi Shirdoust, Taher Azimi, Iraj Sedighi, Mohammad Rahbar, Shahnaz Armin
Abstract
OBJECTIVES: from nine different provinces of Iran. MATERIALS AND METHODS: samples from nine provinces of Iran on a one-year time horizon were gathered. Modified Hedge Test (MHT) and Carba NP-Test were applied to the identification of producing-carbapenemase strains. The most important carbapenemase genes recognized by PCR and gene overexpression of the efflux pump were surveyed by efflux pump inhibitors (EPIs) and confirmed by Real-Time PCR. RESULTS: , respectively. CONCLUSION: may become multi-drug-resistant (MDR) and Extensively Drug-Resistant (XDR) strains and cause a high rate of mortality and morbidity. Thus, it is of necessity to prohibit the spread of antibiotic-resistant strains in hospitals.