Multiphoton characterization and live cell imaging using fluorescent adenine analogue 2CNqA
Jesper R. Nilsson, Carlos Benitez‐Martin, Henry G. Sansom, Pauline Pfeiffer, Tom Baladi, Hoang‐Ngoan Le, Anders Dahlén, Steven W. Magennis, L. Marcus Wilhelmsson
Abstract
(700 nm) = 5.8 GM, 6.8 GM, and 13 GM for the monomer, single-, and double-labelled oligonucleotide, respectively. Using fluorescence correlation spectroscopy, we show that the 2CNqA has a high 2P brightness as the monomer and when incorporated into the ASO, comparing favorably to other FBAs. We furthermore demonstrate the usefulness of the 2P imaging mode for improving detectability of 2CNqA-labelled ASOs in live cells.
Topics & Concepts
FluorescenceNucleobaseCharacterization (materials science)Live cell imagingOligonucleotideFluorescence-lifetime imaging microscopyMonomerChemistryBiological imagingBiophysicsCellMaterials scienceDNANanotechnologyOpticsBiochemistryBiologyPhysicsPolymerOrganic chemistryAdvanced Fluorescence Microscopy TechniquesAdvanced biosensing and bioanalysis techniquesPhotoreceptor and optogenetics research