Green Synthesis of CuO Nanoparticles and its Application on Toxicology Evaluation
Santhoshkumar Jayakodi, Venkat Kumar, M Grigore, E Biscu, A Holban, M Gestal, A Grumezescu, G Grasso, D Zane, R Dragone, A Azam, A Ahmed, M Oves, M Khan, A Memic, M Ponnar, C Thangamani, P Monisha, S Gomathi, K Pushpanathan, S Ammaji, C Sunddepp, Sandeep, Kunchala Ashok, K Praveen, B Murali, P Singh, P Kumar, M Hussain, A Das, G Nayak, S Jillani, M Jelani, N Hassan, S Ahmad, M Hafeez, Synthesis, A Chatterjee, R Sarkar, A Chattopadhyay, P Aich, R Chakraborty, T Basu, G Sharma, N Jasuja, M Kumar, M Ali, S Saran, G Sharma, M Kumar, M Ali, S Vishnu, P Ramaswamy, S Narendhran, R Sivaraj, W Han, J Choi, G Hwang, S Hong, J Lee, S Kang, A Ullah, N Ullah, S Rasheed, H Ullah, M Israr, S Yallappa, J Manjanna, M Sindhe, N Satyanarayan, S Pramod, K Nagaraja, F Kiessling, Z Liu, J Gtjens, D Deryabin, E Aleshina, A Vasilchenko, T Deryabina, L Efremova, I V; Karimov, L Korolevskaya, P Kalatehjari, M Yousefian, M Khalilzadeh, S Palashuddin, U Goswami, S Ghosh, A Chattopadhyay, S Usha, K Ramappa, S Hiregoudar, G Vasanthkumar, D Aswathanarayana, P Ghosh, D Fawcett, S Sharma, G Poinern, G Singaravelu, J Arockiamary
Abstract
This study aims to understand the major effects of Copper oxide nanoparticles on toxic and fish pathogens by using a green method. Safer methods like the usage of microorganisms prove to be eco-friendly and extremely practical than the regular physical and chemical methods. The characters and features of the CuO particles were understood by using analytical techniques like XRD, UV-vis spectroscopy, FTIR, SEM-EDX, TEM and AFM. Further analysis showed that the spherical particle size was around 100 nm. When prepared in pure water to zebrafish, with a 96 h LC50 concentration of 67.61 mg/L. The possibility to evaluate hatching time in the 96h acute embryo toxicity test seems to be an advantage against that of the 96-hour toxicity since this toxicity was a life stage-dependent.