Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles
Huan Zhao, Qifeng Lin, Li Huang, Yunfeng Zhai, Yuan Liu, Yan Deng, Enben Su, Nongyue He
Abstract
, much greater than those achieved by the classical chemiluminescence immunoassay (CLIA, Getein). Moreover, the intra-day variable coefficient can be improved to 1.21-2.12%, and inter-day variability was 2.01-3.49% under the application of magnetic fluorescent beads as an internal standard. The sensitivity and precision have reached a high level, comparable with the current commercial detection kits. The results showed a good correlation with a commercial chemiluminescence assay (CLIA, Abbott), with a correlation coefficient of 0.9883. This proposed method has been successfully applied to the clinical determination of cTnI in the human serum.
Topics & Concepts
MicrosphereChemiluminescenceFluorescenceMagnetic nanoparticlesNanoparticleMaterials scienceImmunoassayLuminescent MeasurementsNanotechnologyChromatographyChemistryChemical engineeringLuminescenceOptoelectronicsOpticsImmunologyAntibodyPhysicsBiologyEngineeringAtomic and Subatomic Physics ResearchLanthanide and Transition Metal ComplexesAdvanced Biosensing Techniques and Applications