The C29–C34 parts of antitumor macrolide aplyronine A serve as versatile actin-affinity tags
Didik Huswo Utomo, Akari Fujieda, Kentaro Tanaka, Momoko Takahashi, Kentaro Futaki, Kenta Tanabe, Hideo Kigoshi, Masaki Kita
Abstract
-methyl enamide showed potent actin-depolymerizing activity. Binding kinetics, molecular docking, and affinity-purification experiments revealed that they are versatile actin-affinity tags to accelerate studies on the mode of action related to cytoskeletal dynamics and the development of PPI-based drug leads.
Topics & Concepts
ChemistryCombinatorial chemistryBiochemistrySynthetic Organic Chemistry MethodsChemical Synthesis and AnalysisMicrobial Natural Products and Biosynthesis