A rapid nucleic acid concentration measurement system with large field of view for a droplet digital PCR microfluidic chip
Jinrong Shen, Jihong Zheng, Zhenqing Li, Yourong Liu, Fengxiang Jing, Xinjun Wan, Yoshinori Yamaguchi, Songlin Zhuang
Abstract
, HEX, ROX and Cy5), and thus it can strengthen the fluorescence intensity for weak signals. The results also demonstrated that the correlation coefficient for the measured concentration and theoretical value was above 99%, by testing the ddPCR products with COVID-19 virus. Such a system can greatly reduce the time required for image acquisition and DNA concentration determination, and thus is able to speed up the lab-to-application process for ddPCR technology.
Topics & Concepts
Digital polymerase chain reactionNucleic acidMicrofluidicsMicrofluidic chipNucleic acid quantitationReal-time polymerase chain reactionChemistryChipSensitivity (control systems)Nucleic acid detectionPolymerase chain reactionNanotechnologyMaterials scienceComputer scienceBiochemistryEngineeringElectronic engineeringGeneTelecommunicationsInnovative Microfluidic and Catalytic Techniques InnovationMicrofluidic and Capillary Electrophoresis ApplicationsElectrowetting and Microfluidic Technologies