Detection of parallel and antiparallel DNA triplex structures in living human cells using in-cell NMR
Tomoki Sakamoto, Yudai Yamaoki, Takashi Nagata, Masato Katahira
Abstract
We introduced oligodeoxynucleotides (ODNs) that form parallel and antiparallel triplex structures in vitro into living human cells and recorded their in-cell NMR spectra. Observation of landmark signals for triplex structures proved for the first time that parallel and antiparallel triplex structures are formed in living human cells.
Topics & Concepts
Antiparallel (mathematics)DNAChemistryCellCrystallographyBiophysicsMaterials scienceBiochemistryBiologyPhysicsMagnetic fieldQuantum mechanicsDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms