Cassava starch-based film infused with biogenic selenium nanoparticles (bio-SeNPs) to reduce foodborne pathogens
Matheus Henrique Nogueira, Luana de Carvalho, André Ricardo Peron dos Santos, Márcia Cristina Furlaneto, Luciana Furlaneto-Maia
Abstract
Abstract The urgent investigation of antimicrobial films is imperative to mitigate the proliferation of pathogens in food, extending product shelf life and ensuring food safety, crucial aspects for public health and the sustainability of the global food system. We developed cassava starch-based films containing 1% and 2% biogenic selenium nanoparticles (Bio-SeNPs) produced by enterocin MF5 to assess antimicrobial activity. The addition of Bio-SeNPs altered the luminosity and gave a reddish hue to the films. UV–visible, FTIR, and thermogram analyses revealed intermolecular interactions between the starch and Bio-SeNPs. Scanning electron microscopy showed a smooth and homogeneous surface of the nanocomposite films. Water vapour permeability ranged from 9.4 to 0.68 g mm h−1 m−2 kPa−1, lower than control values. Swelling values were higher in the film containing 1% Bio-SeNPs, and water vapour transmission was lower. The films exhibited MICs of 5.5 μL mL−1 for Listeria spp., 10.9 μL mL−1 for Salmonella spp., and 250 μL mL−1 for Escherichia coli. Antimicrobial activity ranged from 1.5–1.9 log and 1.6–2.0 log in liquid medium and dry film, respectively, after 24 h. The films were also applied to cherry tomatoes without altering their characteristics compared to the positive control. Our study demonstrated the high potential of using Bio-SeNPs in biodegradable films to prevent food contamination.