High-throughput label-free opioid receptor binding assays using an automated desorption electrospray ionization mass spectrometry platform
Yunfei Feng, Nicolás M. Morato, Kai‐Hung Huang, Mina Lin, R. Graham Cooks
Abstract
The current opioid epidemic has incentivized the discovery of new non-addictive analgesics, a process that requires the screening of opioid receptor binding, traditionally performed using radiometric assays. Here we describe a label-free alternative based on high-throughput (1 Hz) ambient mass spectrometry for screening the receptor binding of new opioid analogues.
Topics & Concepts
ChemistryMass spectrometryElectrospray ionizationChromatographyDesorption electrospray ionizationDesorptionThroughputElectrosprayIonizationAnalytical Chemistry (journal)Chemical ionizationComputer scienceAdsorptionOrganic chemistryIonTelecommunicationsWirelessMass Spectrometry Techniques and ApplicationsPharmacological Effects and AssaysAnalytical Chemistry and Chromatography