Metabolic contrast agents produced from transported solid 13C-glucose hyperpolarized via dynamic nuclear polarization
Andrea Capozzi, Jan Kilund, Magnus Karlsson, Saket Patel, Arthur C. Pinon, François Vibert, Olivier Ouari, Mathilde H. Lerche, Jan Henrik Ardenkjær‐Larsen
Abstract
Abstract Magnetic Resonance Imaging combined with hyperpolarized 13 C-labelled metabolic contrast agents produced via dissolution Dynamic Nuclear Polarization can, non-invasively and in real-time, report on tissue specific aberrant metabolism. However, hyperpolarization equipment is expensive, technically demanding and needs to be installed on-site for the end-user. In this work, we provide a robust methodology that allows remote production of the hyperpolarized 13 C-labelled metabolic contrast agents. The methodology, built on photo-induced thermally labile radicals, allows solid sample extraction from the hyperpolarization equipment and several hours’ lifetime of the 13 C-labelled metabolic contrast agents at appropriate storage/transport conditions. Exemplified with [U- 13 C, d 7 ]-D-glucose, we remotely produce hyperpolarized 13 C-labelled metabolic contrast agents and generate above 10,000-fold liquid-state Magnetic Resonance signal enhancement at 9.4 T, keeping on-site only a simple dissolution device.