Carbon Dots Enable Efficient Delivery of Functional DNA in Plants
Bo Wang, Jian Huang, Mengling Zhang, Yun Wang, Huibo Wang, Yurong Ma, Xiaodong Zhao, Xiao Wang, Changhong Liu, Hui Huang, Yang Liu, Fang Lü, Hengxiu Yu, Mingwang Shao, Zhenhui Kang
Abstract
) leaves and rice roots. Notably, the CDP successfully deliver the hydamycin resistance gene into the rice and induce hygromycin resistance in rice. Also, CDP can achieve the β-glucuronidase gene delivery and expression in mature rice embryo induced callus. The CDP not only facilitates plasmid transport into cells but also protects DNA from DNase degradation. Our work provides a highly efficient DNA delivery system for instant gene expression, which could be a useful and fast gene engineering tool for functional research in plant biology.
Topics & Concepts
Gene deliveryTransfectionBiologyGeneOryza sativaExogenous DNAGene expressionDNAAgrobacteriumGenetically modified ricePlasmidCallusTransgeneCell biologyGenetically modified cropsBotanyGeneticsPlant tissue culture and regenerationCarbon and Quantum Dots ApplicationsAdvanced biosensing and bioanalysis techniques