Role of vitamin D3 on apoptosis and inflammatory-associated gene in colorectal cancer: An in vitro approach
Jisha Elsa Varghese, Shanmugam Velayuthaprabhu, Rengasamy Lakshminarayanan Rengarajan, Arun Meyyazhagan, Vijaya Anand Arumugam, F. Al‐Misned, Hamed A. El‐Serehy
Abstract
The association between vitamin D and cancer has been studied with different focuses. As far as the beneficial effects concern, studies have shown the effects of vitamin D in inflammatory response and oxidative stress in relation with cancer regression. Indirect anti-cancer effects of vitamin D also due to its anti-inflammatory properties. Nuclear factor kappa B (NF-κB) is considered as a major mediator of inflammation and key transcription factor linking inflammation of cancer development. On the other hand, caspase protein also has an important role in apoptosis. Further, apoptosis and the BCL-2 family now inextricably linked to human cancer. Since the vitamin D3 has proven to exert numerous tumor suppressive effects, the relationship of vitamin D3 on the regulation of apoptosis and NF-κB is yet to be studied in detail. Hence, the present study aims at evaluating the efficacy of vitamin D3 on NF-κB and Caspase 3 in the human colorectal carcinoma cell line (HCT116). Treatment of HCT cell line with different concentration of vitamin D3 shown a dose-dependent effect on the expression of Caspase 3 not on NF-κB. This finding suggests that vitamin D does not have any effect on NF-κB, but have a positive effect on Caspase3 on HCT116, which might play a vital role in preventing the colon cancer.