Synthesis and immunological evaluation of synthetic peptide based anti-SARS-CoV-2 vaccine candidates
Qingyu Zhao, Yanan Gao, Min Xiao, Xuefei Huang, Xuanjun Wu
Abstract
For prevention of the coronavirus disease 2019 caused by the novel coronavirus SARS-CoV-2, an effective vaccine is critical. Herein, several potential peptide epitopes from the spike protein of SARS-CoV-2 have been synthesized and covalently linked with the cross-reactive material (CRM197). Immunization of mice with the resulting conjugates induced high titers of IgG antibodies against the spike protein. Importantly, the post-immune sera effectively neutralized SARS-CoV-2 pseudovirus, suggesting the epitopes identified are protective, and these conjugates are promising leads for anti-SARS-CoV-2 vaccine development.
Topics & Concepts
VirologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)PeptideCoronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakChemistryCombinatorial chemistryComputational biologyMedicineBiologyBiochemistryInfectious disease (medical specialty)PathologyOutbreakDiseaseSARS-CoV-2 and COVID-19 Researchvaccines and immunoinformatics approachesImmunotherapy and Immune Responses