Engineered Biosynthesis of 5/5/6 Type Polycyclic Tetramate Macrolactams in an Ikarugamycin (5/6/5 Type)-Producing Chassis
Hong-Bo Jin, Wenjun Zhang, Guangtao Zhang, Liping Zhang, Wei Liu, Changsheng Zhang
Abstract
Combinatorial biosynthesis of 5/5/6 type polycyclic tetramate macrolactams (PoTeMs) was achieved in an engineered ikarugamycin (5/6/5 type) producer by introducing a set of 5/5/6 type PoTeM biosynthetic genes from Streptomyces griseus. This study supports the idea that PoTeMs share a common polyene tetramate precursor generated by the hybrid polyketide synthase/nonribosomal peptide synthetase enzymes. The X-ray crystal structure of pactamide G (7) sets an example for resolving the absolute configuration of 5/5/6 type PoTeMs.
Topics & Concepts
BiosynthesisNonribosomal peptideChemistryPolyenePolyketide synthasePolyketideStreptomyces griseusStereochemistryStreptomycesType (biology)EnzymeBiochemistryBiologyGeneticsBacteriaEcologyMicrobial Natural Products and BiosynthesisEnzyme Production and CharacterizationChemical Synthesis and Analysis