Enhanced Production of Soluble <i>Pyrococcus furiosus</i> α-Amylase in <i>Bacillus subtilis</i> through Chaperone Co-Expression, Heat Treatment and Fermentation Optimization
Kang Zhang, Ruiting Tan, Dongbang Yao, Lingqia Su, Yongmei Xia, Jing Wu
Abstract
α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.
Topics & Concepts
Pyrococcus furiosusBacillus subtilisAmylaseStarchChemistryFermentationBiochemistryIndustrial fermentationThermophileFood scienceBacteriaBiologyEnzymeArchaeaGeneGeneticsEnzyme Production and CharacterizationEnzyme Structure and FunctionProtein purification and stability