Applications of Flow Cytometry in Drug Discovery and Translational Research
Sumana Ullas, Charles Sinclair
Abstract
Flow cytometry is a mainstay technique in cell biology research, where it is used for phenotypic analysis of mixed cell populations. Quantitative approaches have unlocked a deeper value of flow cytometry in drug discovery research. As the number of drug modalities and druggable mechanisms increases, there is an increasing drive to identify meaningful biomarkers, evaluate the relationship between pharmacokinetics and pharmacodynamics (PK/PD), and translate these insights into the evaluation of patients enrolled in early clinical trials. In this review, we discuss emerging roles for flow cytometry in the translational setting that supports the transition and evaluation of novel compounds in the clinic.
Topics & Concepts
DruggabilityFlow cytometryDrug discoveryComputational biologyMass cytometryDrugCytometryPharmacodynamicsBioinformaticsDrug developmentMedicinePharmacologyComputer sciencePharmacokineticsBiologyPhenotypeImmunologyBiochemistryGeneSingle-cell and spatial transcriptomicsCell Image Analysis TechniquesCAR-T cell therapy research