Simple method for transformation and gene editing in medicinal plants
Xuesong Cao, Hongtao Xie, Minglei Song, Lianghui Zhao, Hailiang Liu, Guofu Li, Jian‐Kang Zhu
Abstract
A sample delivery method, modified from cut-dip-budding, uses explants with robust shoot regeneration ability, enabling transformation and gene editing in medicinal plants, bypassing tissue culture and hairy root formation. This method has potential for applications across a wide range of plant species.
Topics & Concepts
Genome editingBiologyAgrobacteriumTransformation (genetics)Regeneration (biology)ShootMedicinal plantsComputational biologyExplant cultureBiotechnologyGeneHairy root cultureGenetically modified cropsBotanyTransgeneCell biologyGeneticsGenomeIn vitroPlant tissue culture and regenerationTransgenic Plants and ApplicationsCRISPR and Genetic Engineering