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Resveratrol protects against ox-LDL-induced endothelial dysfunction in atherosclerosis via depending on circ_0091822/miR-106b-5p-mediated upregulation of TLR4

Jinsong Chen, Yang Liu, Yunyang Liu, Jianye Peng

2022Immunopharmacology and Immunotoxicology12 citationsDOI

Abstract

BACKGROUND: Atherosclerosis (AS) is the most common inducer of cardiovascular diseases, and resveratrol (RSV) has played a protective function in the endothelial injury of AS. This study was to explore the molecular mechanism of RSV in oxidized low-density lipoprotein (ox-LDL)-mediated endothelial dysfunction. METHODS: dual-luciferase reporter assay and RNA pull-down assay. RESULTS: inducing the downregulation of circ_0091822. Circ_0091822 could target miR-106b-5p, and the reversal of circ_0091822 for RSV function was achieved by sponging miR-106b-5p. Circ_0091822 absorbed miR-106b-5p to elevate the level of TLR4. RSV impeded ox-LDL-induced damages by regulating miR-106b-5p/TLR4 axis. CONCLUSION: downregulating circ_0091822 to upregulate miR-106b-5p-related TLR4.

Topics & Concepts

Viability assayDownregulation and upregulationAngiogenesisApoptosisResveratrolChemistryFlow cytometryEndothelial stem cellWestern blotOxidative stressMolecular biologyTLR4Gene knockdownMTT assayInflammationBiologyImmunologyCancer researchBiochemistryIn vitroGeneCircular RNAs in diseasesSirtuins and Resveratrol in MedicineAtherosclerosis and Cardiovascular Diseases