Development of a Multiplex PCR-Based Assay for Rapid Serotyping of <i>Erysipelothrix</i> Species
Yoshihiro Shimoji, Kazumasa Shiraiwa, Haruka Tominaga, Sayaka Nishikawa, Masahiro Eguchi, Hirokazu Hikono, Yohsuke Ogawa
Abstract
The Gram-positive bacterium Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a wide range of mammalian and avian species. Historically, E. rhusiopathiae has been differentiated from other Erysipelothrix species by serotyping. Among 28 serovars of Erysipelothrix species, specific serovars, namely, 1a, 1b, and 2 of E. rhusiopathiae , are associated mainly with the disease in pigs, poultry, and humans; however, other serovar strains are often simultaneously isolated from diseased and healthy animals, indicating the importance of isolate serotyping for epidemiology.
Topics & Concepts
Erysipelothrix rhusiopathiaeSerotypeBiologyMicrobiologyPathogenErysipelasMultiplex polymerase chain reactionVirologyPolymerase chain reactionMedicineGeneticsPathologyGeneMicrobial infections and disease researchStreptococcal Infections and TreatmentsVector-borne infectious diseases