Tissue clearing and 3D imaging in developmental biology
Alba Vieites‐Prado, Nicolas Renier
Abstract
Tissue clearing increases the transparency of late developmental stages and enables deep imaging in fixed organisms. Successful implementation of these methodologies requires a good grasp of sample processing, imaging and the possibilities offered by image analysis. In this Primer, we highlight how tissue clearing can revolutionize the histological analysis of developmental processes and we advise on how to implement effective clearing protocols, imaging strategies and analysis methods for developmental biology.
Topics & Concepts
BiologyClearingGRASPDevelopmental biologyComputational biologyCell biologyComputer scienceEconomicsFinanceProgramming languageCell Image Analysis TechniquesAdvanced Fluorescence Microscopy TechniquesViral Infectious Diseases and Gene Expression in Insects