Zebrafish Embryo Model for Assessment of Drug Efficacy on Mycobacterial Persisters
Susanna Commandeur, Nino Iakobachvili, Marion Sparrius, Mariam Mohamed Nur, Galina V. Mukamolova, Wilbert Bitter
Abstract
Tuberculosis continues to kill millions of people each year. The main difficulty in eradication of the disease is the prolonged duration of treatment, which takes at least 6 months. Persister cells have long been associated with failed treatment and disease relapse because of their phenotypical, though transient, tolerance to drugs. By targeting these persisters, the duration of treatment could be shortened, leading to improved tuberculosis treatment and a reduction in transmission. The unique in vivo environment drives the generation of persisters; however, appropriate in vivo mycobacterial persister models enabling optimized drug screening are lacking.