A stable physisorbed layer of packed capture antibodies for high-performance sensing applications
Lucia Sarcina, Cecilia Scandurra, Cinzia Di Franco, Mariapia Caputo, Michele Catacchio, Paolo Bollella, Gaetano Scamarcio, Eleonora Macchia, Luisa Torsi
Abstract
-related aggregations of antibodies. The long-term stability over two weeks of the physisorbed proteins was also assessed. High-performance sensing was proven by evaluating figures of merit, such as the limit of detection (2 nM) and the selectivity ratio between a negative control and the sensing experiment (0.04), which is the best reported performance for an SPR S1 protein assay. These figures of merit outmatch those measured with more sophisticated biofunctionalization procedures involving chemical bonding of the capture antibodies to the gold surface. The present study opens up interesting new pathways toward the achievement of a cost-effective and scalable biofunctionalization protocol, which could guarantee the prolonged stability of the biolayer and easy handling of the biosensing system.