Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
Jian Zhang, Xin Fang, Yu Mao, Haochen Qi, Jie Wu, Xiaoru Liu, Fangshuo You, Wenci Zhao, Ying Chen, Lei Zheng
Abstract
Abstract Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10 −6 ng/mL in 20 s, with a wide linear range of 10 −5 –10 −1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.