Antibacterial, Antioxidant, and Antidiabetic Activities of TiO2 Nanoparticles Synthesized Through Ultrasonication Assisted Cold Maceration from Stem Extract of Euphorbia hirta
Unknown authors
Abstract
The global scientific community recognizes the green synthesis of nanoparticles due to its environment-friendly approach. In this study, titanium dioxide nanoparticles(TiO2 NPs) have been synthesized by ultrasonication-assisted cold maceration green technique mediated through stem extract of Euphorbia hirta such that the plant's phytochemical constituents are preserved and do not deteriorate. The synthesized TiO2 nanoparticles were characterized by UV spectroscopy, and a sharp peak at 326 nm confirmed the formation of the TiO2 nanoparticles. The surface morphology was analyzed using SEM (Scanning Electron Microscope), which showed that nanoparticles with flakes-like shapes were formed. EDX (Energy Dispersive X-ray Analysis) outcomes have also confirmed the presence of TiO2. The antibacterial activity of the TiO2 nanoparticles was assessed against three bacterial strains (Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa.), with a maximum ZOI of 35±3.5 mm showing that TiO2 nanoparticles have good antibacterial properties. Further antioxidant (DPPH and Phosphomolybdate assay) and antidiabetic activity were evaluated with the IC50 value of 39.01 ± 0.56, 48.29 ± 0.69, and 55 ± 1.69 µg/ml, respectively, which indicated the potency of the synthesized nanoparticles for various biomedical and pharmacological applications.