Molecular dissection of tendon development and healing: Insights into tenogenic phenotypes and functions
Takao Sakai, Ken Kumagai
Abstract
Tendon is a dense connective tissue that transmits contraction forces from skeletal muscles to bones. Adult tendon injury is a significant clinical problem because it occurs frequently with a high recurrence rate, and damaged tendon is rarely restored to full function. The main barrier to improving recovery outcomes is our incomplete understanding of the molecular mechanisms underlying the biological alterations following tendon injury in vivo. In this review, we specifically highlight the cellular dynamism of fibrotic tendon wound healing and the roles of mechanical loading. In particular, we document how tendon stem/progenitor cells expressing the tendon-specific transcription factor Scleraxis (Scx) play a role in fibrotic tendon wound healing, and describe novel experimental systems such as lineage cell tracing and single-cell analysis, both of which can shed light on tendon cell behavior and fate decisions during the tendon wound healing process.