Coadministration of Ginger Roots Extract and Vitamin E Improves Male Fertility of Streptozotocin-Induced Diabetic Rats
Mostafa Abbas, Rehab A. Ghandour, Shimaa R. Emam, Abdel-Azeem As Hegazy, A Ibrahim, K Farrag, A El-Sayed, E, Z Al-Amin, M Thomson, Al Qattan, K, Peltonen Shalaby, R, Ali, Al-Kashef Mm, Al-Qulaly Mm Okasha, M Mohamed, G Hassan, M, R Amann, A Bakr, S Abdelgayed, O El-Tawil, A Bakeer, J Ballester, M Munoz, J Dominguez, T Rigau, J Guinovart, J Rodriguez, S Banihani, S Banihani, E Beach, J Turner, M Bodagh, I Maleki, A Hekmatdoost, P Brezina, N Fahd, F Yunus, Y Zhao, H Chen, M Hardy, I Aniemi, B Zirkin, E Elaraby, N Heba, Hassan Ak, O Abbas, B Eid, H Mosli, M Hany, H El-Bassossy, B Francis, S Sudha, A Isidori, C Pozza, D Gianfrilli, A Isidori, Joshua Jk, Catherine Cl, M Kanter, C Aktas, M Erboga, A Khaki, F Fatemek, N Mohammad, A Amir, C Chelar, N Marefat, H Mohammad, M Khorramabadi, A Talebi, A Sarcheshmeh, A Mirjalili, E Kurutas, S Lenzen, A Lucchesi, N Freitas, L Cassettari, S Marques, C Spadella, D Luis-Rodrguez, A Martnez-Castelao, J Gorriz, F De-Alvaro, Navarro-Gonzalez Jf, Y Majeed, M Shaukat, K Abbasi, M Ahmad, C Maresch, C Stute, G Alves, P Oliveira, D Ktertser, Linn, N Mascolo, R Jain
Abstract
Ginger is an important medicinal plant in folk medicine. Antioxidant, antidiabetic, and anti-inflammatory effects have been reported in ginger extracts. This study was performed to examine how ginger extract (GE) and vitamin E (Vit E) have affected the fertility in Streptozotocin-induced diabetic male rats. Sixty adult male rats were randomly assigned into six groups (n=10). Group 1 served as a negative control, while the other animals were intraperitoneally injected with Streptozotocin (50mg/kg) after fasting for 16 hours to induce diabetes. Group 2 was kept as a diabetic positive control. While groups 3 and 4 were orally given GE alone at 300 and 600mg/kg for 65 consecutive days to cover the whole spermatogenic cycle in rats, respectively. For the same period, groups 5 and 6 received GE (300 and 600mg/kg) plus Vit E (200mg/kg). Serum samples were obtained to estimate blood glucose, insulin, testosterone, FSH, and LH levels. Sex organs have been weighed, besides semen picture and histopathology of the testis were carried out. The results revealed that co-administration of GE and Vit. E increased the fertility index, weights of the testis and epididymis, serum testosterone level and improved semen quality in Streptozotocin-induced diabetic rats. Moreover, co-administration of GE and Vit. E caused hypoglycemia and hypoinsulinemia in administered rats compared to the diabetic positive control. Mild to moderate testicular degeneration and incomplete arrest of spermatogenesis were seen by histopathological examination of diabetic control group. Moreover, GE and Vit. E alleviated testicular lesions induced by diabetes, and improved spermatogenesis in treated groups. Conclusively, diabetic patients who are sexually impotent may benefit from a dietary supplement containing ginger roots and vitamin E.