Development of a Mini-Replicon-Based Reverse-Genetics System for Rice Stripe Tenuivirus
Mingfeng Feng, Luyao Li, Ruixiang Cheng, Yulong Yuan, Yongxin Dong, Minglong Chen, Rong Guo, Min Yao, Yi Xu, Yijun Zhou, Jianxiang Wu, Xin Shun Ding, Xueping Zhou, Xiaorong Tao
Abstract
. Rice stripe virus (RSV) is a monocot-infecting tenuivirus with four negative-stranded/ambisense RNA segments. It is one of the most destructive rice pathogens and causes significant damage to the rice industry in Asian countries. Due to the lack of a reliable reverse-genetics system, molecular characterizations of RSV gene functions and the host machinery underpinning RSV infection in plants are extremely difficult. To overcome this obstacle, we developed a mini-replicon-based reverse-genetics system for RSV in Nicotiana benthamiana. This is the first mini-replicon-based reverse-genetics system for tenuivirus. We consider that this system will provide researchers a new working platform to elucidate the molecular mechanisms dictating segmented tenuivirus infections in plants.