Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
Jonathan A. Robson, Markéta Kubánková, Tamzin Bond, Rian A. Hendley, Andrew J. P. White, Marina K. Kuimova, James D. E. T. Wilton‐Ely
Abstract
A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.
Topics & Concepts
FluorophoreCarbon monoxideViscosityBODIPYFluorescenceChemical engineeringMicroscopyChemistryFluorescence microscopeBiophysicsMaterials scienceNanotechnologyOrganic chemistryCatalysisOpticsBiologyComposite materialPhysicsEngineeringHeme Oxygenase-1 and Carbon MonoxideHemoglobin structure and functionNeonatal Health and Biochemistry