Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody
Javier Macías‐León, Iris A. Bermejo, Alicia Asín, Ana García-García, Ismael Compañón, Ester Jiménez‐Moreno, Helena Coelho, Vincenzo Mangini, Inês S. Albuquerque, Filipa Marcelo, Juan Luis Asensio, Gonçalo J. L. Bernardes, Hiren J. Joshi, Roberto Fiammengo, Ola Blixt, Ramón Hurtado‐Guerrero, Francisco Corzana
Abstract
The molecular basis of antibody 5E5, which recognizes the entire GalNAc unit as a primary epitope is disclosed. The antibody's contacts with the peptide are mostly limited to two residues, allowing it to show some degree of promiscuity. These findings open the door to the chemical design of peptide-mimetics for developing efficient anti-cancer vaccines and diagnostic tools.
Topics & Concepts
ChemistryLectinAntibodyCharacterization (materials science)MUC1Combinatorial chemistryBiochemistryBiophysicsNanotechnologyImmunologyBiologyMaterials scienceMucinGlycosylation and Glycoproteins ResearchMonoclonal and Polyclonal Antibodies ResearchGalectins and Cancer Biology