Sequence Evaluation and Comparative Analysis of Novel Assays for Intact Proviral HIV-1 DNA
Christian Gaebler, Shane D. Falcinelli, Elina Stoffel, Jenna Read, Ross Murtagh, Thiago Y. Oliveira, Víctor Ramos, Julio C. C. Lorenzi, Jennifer Kirchherr, Katherine S. James, Brigitte Allard, Caroline Baker, JoAnn D. Kuruc, Marina Caskey, Nancie M. Archin, Robert F. Siliciano, David M. Margolis, Michel C. Nussenzweig
Abstract
The intact proviral DNA assay (IPDA) and quadruplex PCR (Q4PCR) represent major advances in accurately quantifying and characterizing the replication-competent HIV reservoir. This study compares the two novel approaches for measuring intact HIV proviral DNA in samples from 39 antiretroviral therapy (ART)-suppressed people living with HIV, thereby informing ongoing efforts to deplete the HIV reservoir in cure-related trials.
Topics & Concepts
BiologyDNAGenomeVirologyContext (archaeology)Polymerase chain reactionDNA sequencingGeneticsProvirusVirusSequence (biology)Sequence analysisHuman immunodeficiency virus (HIV)Computational biologyMolecular biologyGenePaleontologyHIV Research and TreatmentHIV/AIDS drug development and treatmentHIV/AIDS Research and Interventions