Sabatier Principle for Rationalizing Enzymatic Hydrolysis of a Synthetic Polyester
Jenny Arnling Bååth, Kenneth Jensen, Kim Borch, Peter Westh, Jeppe Kari
Abstract
at the maximum. LCC on the other hand had an intrinsic substrate affinity closer to the Sabatier optimum, and the turnover rate was 5-fold improved at weakened substrate binding. Our results showed that the Sabatier principle may indeed rationalize enzymatic PET degradation and support process optimization. Finally, we suggest that future discovery efforts should consider enzymes with weakened substrate binding because strong adsorption seems to limit their catalytic performance.
Topics & Concepts
PolyesterEnzymatic hydrolysisHydrolysisChemistryEnzymeBiochemical engineeringCombinatorial chemistryOrganic chemistryEngineeringbiodegradable polymer synthesis and propertiesMicroplastics and Plastic PollutionEnzyme Catalysis and Immobilization