A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types
Miriam E. Hurley, Thomas M. D. Sheard, Ruth Norman, Hannah M. Kirton, Shihab Shah, Eleftheria Pervolaraki, Zhaokang Yang, Nikita Gamper, Ed White, Derek S. Steele, Izzy Jayasinghe
Topics & Concepts
CorrelativeTotal internal reflection fluorescence microscopeRyanodine receptorSecond messenger systemSignallingSuperresolutionCalcium signalingLive cell imagingFörster resonance energy transferFluorescence-lifetime imaging microscopyBiologyBiophysicsComputer sciencePattern recognition (psychology)Cell biologyIntracellularPhysicsFluorescenceArtificial intelligenceImage (mathematics)CellOpticsBiochemistryPhilosophyLinguisticsAdvanced Fluorescence Microscopy TechniquesCell Image Analysis TechniquesAdvanced Electron Microscopy Techniques and Applications