Decolourization of Dye Wastewater by A Malaysian isolate of Aspergillus iizukae 605EAN Strain: A Biokinetic, Mechanism and Microstructure Study
Efaq Ali Noman, Adel Al‐Gheethi, Balkis A. Talip, Radin Maya Saphira Radin Mohamed, Amir Hashim Mohd Kassim
Abstract
The current study aimed to investigate the potential of Aspergillus iizukae EAN605 for removing remazol brilliant blue R (RBBR), methyl orange (MO) and methyl red (MR) from dye-containing wastewater to provide controlled degradation pathway for the dyes in the wastewater. The removal rate and batch kinetic coefficients of the dye, as well as the microstructure of A. iizukae EAN605 mycelium during the mycoremediation process, were investigated in the Bach study. The decolourisation was due to the action of laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP). The maximum removal of RBBR was 94.78% at 10 mg/100 mL observed after 9 days. Meanwhile, the removal of MO and MR was 84.90% and 47.65% at 40 mg/100 mL observed after 3 days of experiment. The kinetic coefficients for the removal of RBBR, MO and MR by A. iizukae EAN605 were determined as K = 1032.21 mg log10 cell mL–1 d–1 and Ks = 65,637.90 mg L–1 for RBBR; K = 4.64 mg log10 cell mL–1 d–1 and Ks = 460.16 mg L–1 for MO; and K = 0.576 mg log10 cell mL–1 d–1 and Ks = 228.82 mg L–1 for MR. The Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy (FESEM-EDX), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and Raman Spectroscopy analysis confirmed that the biosorption of dyes destroyed the mycelium surface and changed its functional groups structure. The findings have revealed that the high potential of A. iizukae EAN605 in dyes decolourisation.