A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section <i>Flavi</i> Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger
Perng‐Kuang Chang
Abstract
species and aspergilli in other sections ("section" is a taxonomic rank between genus and species). This cross-Aspergillus section system is for wild-type isolates and does not require homologous donor DNAs to be added, NHEJ-deficient strains to be created, or forced recycling of knockout recipients to be performed for multiple-gene targeting. Hence, it simplifies and expedites the gene-targeting process significantly.
Topics & Concepts
CRISPRBiologyAspergillus nidulansGeneticsAspergillus flavusHygromycin BGene knockoutGeneGenome editingLocus (genetics)Cas9Transformation (genetics)MicrobiologyMutantCRISPR and Genetic EngineeringPlant-Microbe Interactions and ImmunityFungal and yeast genetics research