Peroxidasin-mediated bromine enrichment of basement membranes
Cuiwen He, Wenxin Song, Thomas A. Weston, Caitlyn Tran, Ira Kurtz, Jonathan E. Zuckerman, Paul Guagliardo, Jeffrey H. Miner, Sergey V. Ivanov, Jeremy Bougoure, Billy G. Hudson, Selene Colon, Paul Voziyan, Gautam Bhave, Loren G. Fong, Stephen G. Young, Haibo Jiang
Abstract
Br) in basement membranes of normal human and mouse kidneys. In peroxidasin knockout mice, bromine enrichment of basement membranes of kidneys was reduced by ∼85%. Proteomic studies revealed bromination of tyrosine-1485 in the NC1 domain of α2 collagen IV from kidneys of wild-type mice; the same tyrosine was brominated in collagen IV from human kidney. Bromination of tyrosine-1485 was reduced by >90% in kidneys of peroxidasin knockout mice. Thus, in addition to promoting sulfilimine cross-links in collagen IV, peroxidasin can also brominate a bystander tyrosine. Also, the fact that bromine enrichment is largely confined to basement membranes implies that peroxidasin activity is largely restricted to basement membranes in mammalian tissues.