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Isolation and Characterization of a Novel Laccase for Lignin Degradation, LacZ1

Weiran Zhang, Weiwei Wang, Weiwei Wang, Jinghong Wang, Guinan Shen, Yuan Yuan, Lei Yan, Hongzhi Tang, Weidong Wang, Weidong Wang

2021Applied and Environmental Microbiology59 citationsDOIOpen Access PDF

Abstract

Lignin is a natural aromatic polymer that is not easily degraded, hindering the efficient use of lignocellulose-rich biomass resources, such as straw. Biodegradation is a method of decomposing lignin that has recently received increasing attention. In this study, we screened a gene encoding laccase from the lignocellulose-degrading microbial consortium WSC-6, purified the corresponding protein LacZ1, characterized the enzymatic properties of laccase LacZ1, and speculated that the degradation pathway of LacZ1 degrades lignin. This study identified a new, high-temperature bacterial laccase that can degrade lignin, providing insight into lignin degradation by this laccase.

Topics & Concepts

LigninLaccaseGel permeation chromatographySize-exclusion chromatographyChemistryChromatographyBiochemistryOrganic chemistryEnzymePolymerEnzyme-mediated dye degradationMicrobial Metabolism and ApplicationsLignin and Wood Chemistry
Isolation and Characterization of a Novel Laccase for Lignin Degradation, LacZ1 | Litcius