Adjustment of Cell Adhesion on Polyurethane Structures via Control of the Hard/Soft Segment Ratio
Gaëtan Lutzweiler, Julien Barthès, Nihal Engin Vrana, Michel Rawiso, Benoît Louis, Josselin Mayingi, Albane Carre, Wiebke Drenckhan, Pierre Schaaf
Abstract
Abstract Creating substrates with a similar composition that can either prevent or promote cell adhesion is still a challenging feat. Here, it is shown that a strikingly simple method of tuning the amount of hard segments or isocyanate index (NCO ind ) of a polyurethane (PU) film allows to modulate cell adhesiveness. PU films are synthesized with NCO ind of 75, 100, 200, 300 and 400 corresponding to ratios of isocyanate to hydroxyl functions of 0.75, 1, 2, 3, 4, respectively. The adhesive capacity of NIH 3T3 fibroblasts (3T3) and Wharton's jelly mesenchymal stem cells (WJMSCs) are dependent on the NCO ind . For NCO ind below 300, no cell adhesion can be observed regardless of the cell type, whereas for NCO ind of 300 and 400 cells adhere to the PU surface. WAXS and small angle X‐ray scattering (SAXS) studies reveal that variations of NCO ind allows to modulate the phase separation in PU films. Porod's law shows that for NCO ind of 300 and 400, the hard–soft segment interface is sharp. Conversely, samples with smaller NCO ind present diffuse interfaces. Hence, the morphology of the interface between hard and soft domains appears to be a critical feature that correlates with the adhesion capacity of cells.