Whole-Genome Sequencing-Based Re-Identification of <i>Pseudomonas putida</i> / <i>fluorescens</i> Clinical Isolates Identified by Biochemical Bacterial Identification Systems
Mari Tohya, Kanae Teramoto, Shin Watanabe, Tomomi Hishinuma, Masahito Shimojima, Miho Ogawa, Tatsuya Tada, Yoko Tabe, Teruo Kirikae
Abstract
Most of the clinical isolates, identified as P. putida or P. fluorescens, were misidentified in clinical laboratories. Whole-genome sequencing (WGS) revealed that these isolates belonged to different Pseudomonas species, including novel species. WGS is a gold-standard method to identify Pseudomonas species, and MALDI-TOF MS analysis has the potential to complement WGS to reliably identify them.
Topics & Concepts
Pseudomonas fluorescensPseudomonas putidaBiologyPseudomonasWhole genome sequencingPseudomonas aeruginosaMicrobiologyGenomeGeneticsComputational biologyBacteriaGeneGenomics and Phylogenetic StudiesPlant Pathogenic Bacteria StudiesInfections and bacterial resistance