Binding of exogenous cyanide reveals new active-site states in [FeFe] hydrogenases
Maria Alessandra Martini, Konstantin Bikbaev, Yunjie Pang, Christian Lorent, Charlotte Wiemann, Nina Breuer, Ingo Zebger, Serena DeBeer, Ingrid Span, Ragnar Björnsson, James A. Birrell, Patricia Rodríguez‐Maciá
Abstract
-bound states can also be generated in wild-type hydrogenases, but do not form as readily as with the Cys to Ala variants. These results highlight how the interaction between the first amino acid in the proton transfer pathway and the active site tunes ligand binding to the open coordination site and affects the electronic structure of the H-cluster.
Topics & Concepts
HydrogenaseActive siteCyanideChemistryAcceptorBinding siteCofactorPhotochemistryInorganic chemistryCatalysisEnzymeBiochemistryPhysicsCondensed matter physicsMetalloenzymes and iron-sulfur proteinsElectrocatalysts for Energy ConversionHydrogen Storage and Materials