Validation of transparent and flexible neural implants for simultaneous electrophysiology, functional imaging, and optogenetics
Lina Koschinski, Bohdan Lenyk, Marie Jung, Irene Lenzi, Björn M. Kampa, Dirk Mayer, Andreas Offenhäusser, Simon Musall, Viviana Rincón Montes
Abstract
a combination of solid gold interconnects and PEDOT:PSS-based electrodes (pp-μECoGs). Both implants allowed simultaneous multimodal recordings but pp-μECoGs offered the best overall electrical, electrochemical, and optical properties with negligible photo-induced artefacts to light wavelengths of interest. Showing functional chronic stability for up to four months, pp-μECoGs also allowed the simultaneous functional mapping of electrical and calcium neural signals upon visual and tactile stimuli during widefield imaging. Moreover, recordings during two-photon imaging showed no visible signal attenuation and enabled the correlation of network dynamics across brain regions to individual neurons located directly below the transparent electrical contacts.