High‐Level Production of Phenylacetaldehyde using Fusion‐Tagged Styrene Oxide Isomerase
Joel P. S. Choo, Richard A. Kammerer, Xiaodan Li, Zhi Li
Abstract
Abstract An order‐of‐magnitude improvement in the production of phenylacetaldehyde to 3.37 M (405 g L −1 ) from the enzymatic isomerisation of styrene oxide was achieved. A small ubiquitin‐related modifier (SUMO) tag increases the productivity of the whole‐cell biocatalytic system by enhancing the expression of active membrane‐bound styrene oxide isomerase (SOI) while retaining enzyme catalytic efficiency and broad natural substrate scope. The isomerisation was performed by using Escherichia coli expressing SUMO‐tagged SOI in an organic‐aqueous biphasic system to yield 96% of phenylacetaldehyde. magnified image
Topics & Concepts
ChemistryStyrene oxidePhenylacetaldehydeStyreneIsomerizationSubstrate (aquarium)Yield (engineering)CatalysisOrganic chemistryCopolymerPolymerMaterials scienceMetallurgyGeologyOceanographyEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionPancreatic function and diabetes