Growth conditions trigger genotype-specific metabolic responses that affect the nutritional quality of kale cultivars
Hirofumi Ishihara, Sara Alegre, Jesús Pascual, Andrea Trotta, Wei Yang, Baoru Yang, Fatemeh Seyednasrollah, Meike Burow, Saijaliisa Kangasjärvi
Abstract
Kales (Brassica oleracea convar. acephala) are fast-growing, nutritious leafy vegetables ideal for year-round indoor farming. However, selection of the best cultivars for growth under artificial lighting necessitates a deeper understanding of leaf metabolism in different kale types. Here we examined a curly-leaved cultivar, Half Tall, and a lacinato-type cultivar, Black Magic, under moderate light (130 µmol photons m-2 s-1/22 °C) and high light (800 µmol photons m-2 s-1/26 °C) conditions. These conditions induced genotype-dependent differences in nutritionally important metabolites, especially anthocyanins and glucosinolates (GSLs), in kale cultivars. In the pale green Half Tall, growth under high light conditions did not induce changes in either pigmentation or total GSL content. In contrast, the purple pigmentation of Black Magic intensified due to increased anthocyanin accumulation. Black Magic showed reduced contents of indole GSLs and increased contents of aliphatic GSLs under high light conditions, with notable cultivar-specific adjustments in individual GSL species. Correlation analysis of metabolite profiles suggested cultivar-specific metabolic interplay between serine biosynthesis and the production of indole GSLs. RNA sequencing identified candidate genes encoding metabolic enzymes and regulatory components behind anthocyanin and GSL biosynthesis. These findings improve our understanding of leaf metabolism and its effects on the nutritional quality of kale cultivars.