Extracellular vesicles transfer chromatin-like structures that induce non-mutational dysfunction of p53 in bone marrow stem cells
Jamal Ghanam, Venkatesh Kumar Chetty, Srishti Anchan, Laura Reetz, Qiqi Yang, Emeline Rideau, Xiaomin Liu, Ingo Lieberwirth, Anna Wrobeln, Peter F. Hoyer, Dirk Reinhardt, Basant Kumar Thakur
Abstract
Small extracellular vesicle (sEV)-DNA has recently emerged as a promising biomarker for cancer diagnosis and prognosis 1 . Despite the growing interest in EV-DNA, many questions related to its nature, loading mechanism, localization, and post-shedding function(s) remain unrevealed. Recently, we have published evidence suggesting an unequal distribution of sEV-DNA between different compartments of the recipient cells, including the nucleus 2 . This finding motivated us to ask whether sEV-DNA is associated with proteins and what is the consequence of this association in the recipient cells. Although histones are abundant in sEVs 3 , whether they are free or associated with sEV-DNA and what is the effect of this association is unknown.