Overview of Analytical Methods for Characterizing the Charge Heterogeneity of Antibody-Drug Conjugates
Ayat Abbood
2024International Journal of Advanced Pharmaceutical Sciences and Research17 citationsDOIOpen Access PDF
Abstract
Antibody-drug conjugates (ADCs) are ideal candidates for selective therapy. ADCs display considerable heterogeneity resulting from various modifications. One of the molecular characteristics of ADCs is charge heterogeneity. Several analytical methods could be used to characterize the charge heterogeneity of ADC including ion exchange chromatography (IEC), isoelectric focusing (IEF), and capillary electrophoresis (CE). This work aimed to give insights into the different analytical techniques used to determine pI and the charge distribution profile of ADCs.
Topics & Concepts
DrugConjugateAntibody-drug conjugateAntibodyChemistryComputational biologyPharmacologyMedicineMonoclonal antibodyBiologyImmunologyMathematicsMathematical analysisHER2/EGFR in Cancer ResearchEstrogen and related hormone effectsRadiopharmaceutical Chemistry and Applications