Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry
Wei-Yuan Hsieh, Kevin J. Williams, Baolong Su, Steven J. Bensinger
Abstract
Immune cells, such as macrophages, reprogram their lipid metabolism in response to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and cytokine receptors (e.g., interferons, interleukins). Profiling these changes can be achieved with shotgun mass spectrometry. This protocol provides step-by-step instructions on the generation and stimulation of bone marrow-derived macrophages (BMDMs), sample collection, and lipid extraction for profiling the macrophage lipidome. For complete details on the use and execution of this protocol, please refer to Hsieh et al. (2020).
Topics & Concepts
LipidomeShotgunS1PR1ReceptorProfiling (computer programming)MacrophageImmune systemComputational biologyCytokineBiologyLipidomicsChemistryImmunologyCell biologyBioinformaticsComputer scienceCancer researchBiochemistryGeneIn vitroOperating systemVascular endothelial growth factor AVascular endothelial growth factorVEGF receptorsMetabolomics and Mass Spectrometry StudiesImmune Response and InflammationPeroxisome Proliferator-Activated Receptors