NIR‐II Ratiometric Chemiluminescent/Fluorescent Reporters for Real‐Time Monitoring and Evaluating Cancer Photodynamic Therapy Efficacy
Lichao Su, Yi‐Ming Chen, Hongqi Huo, Naishun Liao, Ying Wu, Xiaoguang Ge, Zhiyong Guo, Zhongxiang Chen, Xuan Zhang, Jibin Song
Abstract
Abstract The development of probes for early monitoring tumor therapy response may greatly benefit the promotion of photodynamic therapy (PDT) efficacy. Singlet oxygen ( 1 O 2 ) generation is a typical indicator for evaluating PDT efficacy in cancer. However, most existing probes cannot quantitatively detect 1 O 2 in vivo due to the high reactivity and transient state, and thus have a poor correlation with PDT response. Herein, a 1 O 2 ‐responsive theranostic platform comprising thiophene‐based small molecule (2SeFT‐PEG) and photosensitizer Chlorin e6 (Ce6) micelles for real‐time monitoring PDT efficacy is developed. After laser irradiation, the Ce6‐produced 1 O 2 could simultaneously kill cancer and trigger 2SeFT‐PEG to produce increased chemiluminescence (CL) and decreased fluorescence (FL) signals variation at 1050 nm in the second near‐infrared (NIR‐II, 950–1700 nm) window. Significantly, the ratiometric NIR‐II CL/FL imaging at 1050 nm could effectively quantify and monitor the concentration of 1 O 2 and O 2 consumption or recovery, so as to evaluate the therapeutic efficacy of PDT in vivo. Hence, this 1 O 2 activated NIR‐II CL/FL probe provides an efficient ratiometric optical imaging platform for real‐time evaluating PDT effect and precisely guiding the PDT process in vivo.