Rapid Detection of <i>Clostridium tetani</i> by Recombinase Polymerase Amplification Using an Exo Probe
Mingjing Guo, Feng Pan, Liqun Zhang, Chunfeng Feng, Jie Fu, Xiaoyun Pu, Fei Liu
Abstract
detected in less than 20 min, with a detection limit of 20 copies/reaction. The RPA assay displayed good selectivity, and there were no cross-reactions with other infectious bacteria common in penetrating wounds. Tests of target-spiked serum and pus extract revealed that RPA is robust to interfering factors and has great potential for further development for biological sample analysis. This method has been confirmed to be reliable for discriminating between toxic and nontoxic C. tetani strains. The RPA assay dramatically improves the diagnostic efficacy with simplified device architecture and is a promising alternative to real-time PCR for tetanus detection.
Topics & Concepts
Clostridium tetaniRecombinase Polymerase AmplificationAmpliconPolymerase chain reactionRecombinaseBiologyClostridium botulinumMicrobiologyClostridiaceaeNeurotoxinBacteriaVirologyTetanusGeneToxinGeneticsBiochemistryRecombinationVaccinationBacterial Identification and Susceptibility TestingStreptococcal Infections and TreatmentsAntimicrobial Resistance in Staphylococcus